Doctor Vida is a portable, handheld device for isothermal nucleic acid amplification of viral genetic biomarkers.

It is operated from any smartphone in a clinical or research setting, but also at home, at the office, etc.

The validated sample types include nasal or nasopharyngeal swabs, collected in any viral preservation medium.

There is almost no sample preparation, other than collecting the swab sample into the resuspension medium and pipetting a few ul of the sample into a single-use reaction tube, a 5-10 minute preparation time. No RNA extraction and purification is needed.

Doctor Vida is a multi use pocket analyser that runs single samples, with low risk of contamination

Storage of these tubes requires refrigeration, and only a small time is needed to equilibrate to room temperature.

Time to result between 20 and 50 minutes max, and delivered directly to patient’s smartphone.

After running for a period of 20 - 50 min, depending on the viral load, the isothermal PCR will detect viral RNA in the test tube if present, by targeting its N gene and amplifying it exponentially. This will be reported as a positive result. If no virus is present the device will report a negative test result.

The higher the specimen’s viral load, the sooner the CT will be triggered. By sending the PCR data to the smartphone by bluetooth, a graph will will show the intensity of amplification, or no amplification of the N gene inside the tube.